2020 Fiscal Year Final Research Report
Mechanochemical tuning of artificial mechanosensitive channels at the two-dimensional interface
| Project/Area Number |
19K20404
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 62010:Life, health and medical informatics-related
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2021-03-31
|
| Keywords | DNAナノテクノロジー / メカノケミストリー / 機械受容チャネル / 界面膜 / 力学的チューニング |
|---|
| Outline of Final Research Achievements |
The purpose of this study is to artificially construct a molecular conversion system accompanied with the drive of mechanosensitive channels by external mechanical stimuli. Using the structural flexibility of single-stranded DNA and the rigidity of the double helix, DNA nanochannels were designed and created by DNA origami. Transmission electron microscopy visually showed that the DNA nanochannels were softly deformed in aqueous solution, which suggested that it could be deformed by external mechanical force. In association with this study, the DNA nanoplates amphiphilized by the introduction of a partially hydrophobic group led to the formation of water-in-oil droplet capsules and ion transport between the capsules.
|
| Free Research Field |
界面科学
|
| Academic Significance and Societal Importance of the Research Achievements |
力学的刺激の機械受容チャネルを介した生体に対する作用の解明が，「メカノバイオロジー」という学問領域として進められている．より体系的かつ物理的に力学的刺激が生体に与える影響を調査し，理学・作業療法，医学応用への科学的基盤を強固にするためには，誰にでも扱えるシンプルなモデルを無生物でin vitroに構築することが重要である．本研究で提示するDNAナノチャネルは細胞膜中の機械受容チャネルの力学応答を二次元界面膜という無生物構造体で再現することを可能にし，細胞の力覚応答機構の解明に貢献する．
|
