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2020 Fiscal Year Final Research Report

Quantitative elucidation of inflammation-inducing mechanism based on membrane softening

Research Project

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Project/Area Number 19K20665
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionSaitama University (2020)
Tokyo Medical and Dental University (2019)

Principal Investigator

Matsuzaki Takahisa  埼玉大学, 理工学研究科, 助教 (50830527)

Project Period (FY) 2019-04-01 – 2021-03-31
Keywords光技術 / 細胞膜 / 硬さ / イメージング / 発生 / 疾病 / 臓器
Outline of Final Research Achievements

Recently, mechanical properties of the cell membrane is involved in the initial inflammatory reaction of the liver steatosis.Since there was no human liver organoid model in which multiple types of cells were reconstructed, the question "Can softening of the cell membrane induce inflammation of the human liver?" remains unsolved. In this study, I constructed a microscopic system to visualizes the softening process of the cell membrane during inflammatory process of liver organoid. Interestingly, we clearly visualized the membrane of liver organoid rapidly started to soften after the addition of inflammation inducer.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

肝臓の炎症メカニズムに関しては分子生物学的な研究が先行しており、毒(細菌成分や過剰のアルコール・脂肪)が細胞膜のタンパク質に結合して起こる緩やかな反応に着目が置かれている。しかし、ヒトの肝臓モデルが無かったため、既存研究は単一種の細胞に強く依存して行われていた。一方で本研究ではヒトのミニ肝臓を対象に、構築した顕微鏡システムを用いて細胞膜の硬さの精密測定が可能である。これによって生物学的なアプローチだけでは見えてこなかった炎症・線維化のメカニズムを物理化学的なアプローチからも切り込み、新規炎症メカニズムの解明につながる重要な課題と考えられる。

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Published: 2022-01-27   Modified: 2023-01-30  

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