2020 Fiscal Year Annual Research Report
Mechanistic basis of new generation laminin (NGL) for developing paraxial mesoderm cell lineages from hiPSCs
Project/Area Number |
19K20671
|
Research Institution | Kyoto University |
Principal Investigator |
Zhao Mingming 京都大学, iPS細胞研究所, 特定研究員 (50754206)
|
Project Period (FY) |
2019-04-01 – 2021-03-31
|
Keywords | 次世代ラミニン / iPS細胞 / 骨格筋幹細胞 / 再生医学 |
Outline of Annual Research Achievements |
By using the new generation laminin (NGL), which was conjugated heparan sulfate chains (HS) to Laminin-E8 fragments, we established a Xeno-free system for differentiating myocytes and muscle stem cells from hiPSCs. In the step-wised protocol, NGL time-dependently increases the populations of primitive streak, paraxial mesoderm, dermomyotome, muscle progenitors, myocytes, and then muscle stem cells. The mechanism is that HS bind to FGF2 in the culture medium, form a stable high affinity HS-FGF2-FGFR complexes on cell surface, then strongly stimulate FGFR signaling pathway at the starting point of cell differentiation. These effects depend on the unique structure of NGL, that could not be replaced by treating with high dose of FGF2 or coating with the mixture of laminin-E8 fragments and HS.
|
Research Products
(4 results)
-
-
[Journal Article] Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model2020
Author(s)
Mingming Zhao, Atsutoshi Tazumi, Satoru Takayama, Nana Takenaka-Ninagawa, Minas Nalbandian, Miki Nagai, Yumi Nakamura, Masanori Nakasa, Akira Watanabe, Makoto Ikeya, Akitsu Hotta, Yuta Ito, Takahiko Sato, and Hidetoshi Sakurai
-
Journal Title
Stem Cell Reports
Volume: 15
Pages: 80-94
DOI
Peer Reviewed / Open Access
-
-