2019 Fiscal Year Research-status Report
Micro-scale, in-vivo structural and functional tomography of rodent habenula using polarization-sensitive optical coherence tomography
| Project/Area Number |
19K20674
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| Research Institution | Hiroshima University |
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Principal Investigator |
カサラゴッド デイーパカマス 広島大学, 医系科学研究科(医), 助教 (40773908)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | optical imaging |
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| Outline of Annual Research Achievements |
Recent neuroscience research have shown evidences of habenula as a critical neuroanatomical hub that connects and regulates pathways associated with a range of behaviours including reproductive behaviours, central pain processing, nutrition, sleep-wake cycles, stress responses, and learning with lateral and medial habenula showing differences in connectivity and function. This research study aimed to develop a deep tissue volume optical imaging tool based on polarization sensitive-optical coherence tomography(PS-OCT)for small animal brain imaging in-vivo. The progress of the research activity so far is as follows: 1. A prototype design of polarization-sensitive optical coherence tomography (PS-OCT) was attempted using deep wavelength light source at 1.7 micron specifically for brain imaging. However, the optical design had to be slightly altered due to the low light coupling efficiency into the detectors with the polarization-sensitive detection. The development work is ongoing. 2. Owing to the slight delay in the OCT imaging development, in parallel a novel 3D microscope based on fluorescence was built and successfully demonstrated that would provide structural and functional information of habenula as well as whole brain but complementary to OCT. The novel microscope was built using deep ultraviolet light combining surface excitation property with block-face imaging.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
A prototype design of polarization-sensitive optical coherence tomography (PS-OCT) was attempted. However, there were issues with regard to low light coupling into the detectors with polarization-sensitive detection.
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| Strategy for Future Research Activity |
Building of the OCT set-up with a revised plan without the polarization-sensitive detection is ongoing. In parallel, optimization of the novel 3D fluorescence microscope developed that employs single wavelength of illumination for multiple fluorescent probes is also ongoing .
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| Causes of Carryover |
As discussed in the research achievements, a set-up in the initial plan of the PS-OCT development has called for revising the engineering design of the OCT setup. The amount unused in the previous fiscal year shall be used mainly towards the development of simple OCT system using long wavelength laser source.
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