Elucidation of bone formation behavior on the surface of octacalcium phosphate with different spatial distribution of bone forming factor/polysaccharide complex

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K21104
• Project/Area Number (Other): 18H05938 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0401:Materials engineering, chemical engineering, and related fields
• Research Institution: Tohoku University
• Principal Investigator: Hamai Ryo 東北大学, 歯学研究科, 助教 (00824004)
• Project Period (FY): 2018-08-24 – 2021-03-31
• Keywords: 人工骨補填材 / 表面化学修飾 / リン酸八カルシウム / グリコサミノグリカン / タンパク質

Outline of Final Research Achievements

Present study examined that the conditions for constraction of models to examine the relation between spatial distribution of bone morphological protein (BMP) /glycosaminoglycan (GAG) complex from the surface of octacalcium phosphate (OCP) and bone formation induced by osteoblastic cells. Chondroitin sulfate was selected as GAG. Model protein of BMP was applied in this study. The results suggest that the release behavior of model protein/GAG complex from the surface of OCP was regulated through the control of the binding strength between the chondroitin sulfate and amino groups modified on OCP. It is expected that the surface model established based on the finding of this study could contribute to the fabrication of bone substitute showing higher bone regeneration ability by mimicking storage of BMP in the bone tissue and stimulation mechanism of undifferentiated mesenchymal stem cells by BMP via GAG.

Free Research Field

生体材料工学

Academic Significance and Societal Importance of the Research Achievements

骨組織に含まれる多糖類であるグリコサミノグリカンと骨形成に寄与するタンパク質（BMP）の親和性に着目し，それらの複合体とリン酸八カルシウム（OCP）表面との化学結合の強さが異なる表面モデルを構築する条件を見出した。OCPは，骨補填材（人工骨）として応用されている材料である。本モデルを用いることで，骨修復材料の表面から周囲にかけてのBMPと複合化したグリコサミノグリカンの存在量の密度が骨組織を形成する細胞の活性化に及ぼす影響の解明に寄与できると考えられる。これにより，骨再生能の向上に最適な方法でBMPのみならず，関連組織の再生を促すタンパク質を担持した骨補填材開発への応用を拓くものである。