2018 Fiscal Year Annual Research Report
Study on the Influence of Environmental Concentration of Ozone on Lung and Skin Cells by Lipid Analysis
| Project/Area Number |
18H05968
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| Allocation Type | Single-year Grants |
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| Research Institution | Hokkaido University |
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Principal Investigator |
喬 琳 北海道大学, 保健科学研究院, 助教 (30821524)
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | ozone / lipid oxidation / lung / lipidomic / ozonation / LC/MS |
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| Outline of Annual Research Achievements |
Ozone is a universal air pollutant with extremely high oxidative activity. The livings on the earth are hard to avoid the deleterious effect of ozone, because many organs, such as the respiratory airway and the skin, expose to the air directly. This study aims to set up a novel lipidomic methodology to detect the oxidized lipids during exposure to ambient level of ozone by high resolution LC/MS.
The main achievements of the first year are summaries as below:
(1) We studied the model lipid membrane system which is composed by the main components of the cell membrane, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol. We successfully identified and quantified several kinds of ozonation products. We also found mixing cholesterol into the membrane can strongly influence the amount of certain ozonation products.
(2) We successfully set up a culture system with lung cell and ozone which is used to mimic the lung under oxidation of low-level ozone. We also identified some aldehyde from certain unsaturated lipids of the cultured cell as oxidation products after the ozone exposure.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We successfully got the aim of the first year.
Three steps were proposed for the present research and half of them were finished including the study of the model lipid membrane and the pre-experiment of stablishing a lipidomic methodology for the lung cell when exposed to the ambient level of ozone. The ozonized lipids were successfully identified. The results of the model lipid membrane are under summarizing and will be submit to the academical journal soon. The lipidomic methodology will be further optimized and utilized in the quantification of the ozonized lipids.
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| Strategy for Future Research Activity |
In the next year, the plans are as follow:
1. The lipidomic methodology by LC/MS will be further optimized and utilized to quantifying the oxidized lipids.
2. The influencing factor of the oxidation reaction between lung cell and ozone will be further studied including the ozone concentration, the exposure time and the humility of the air.
3. The mechanism of this oxidation process will be further investigated by combining with other biochemical methods.
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