2019 Fiscal Year Final Research Report
Study on the Influence of Environmental Concentration of Ozone on Lung and Skin Cells by Lipid Analysis
| Project/Area Number |
19K21122
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| Project/Area Number (Other) |
18H05968 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
Qiao Lin 北海道大学, 保健科学研究院, 助教 (30821524)
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | low-level ozone / ozonation / lipid oxidation / A549 cell / lung surfactant / lung damage / orbitrap LC-MS/MS |
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| Outline of Final Research Achievements |
Ozone, with extremely high oxidative reactivity, is a common environmental toxicant to the individuals. The respiratory airways and skin tissues are two major organs most directly exposed to this oxidant pollutants. Ozone is hypothesized to initiate intracellular oxidative stress and induce the inflammation in the lung and skin through the formation of oxidized lipids. In the present study, in order to study the lipid oxidation during the exposure to the low-level ozone, which is similar to the average concentration observed in ambient air, we successfully establish a novel and sensitive lipid analysis method by high performance liquid chromatography combined with high-resolution orbitrap tandem mass spectrometry. The ozonized lipids of the lung surfactants and the lung cells were successfully identified and quantified. It helps us to further understand the reaction mechanism between the lipid in the lung and the low-level ozone in the air at a molecular level.
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| Free Research Field |
化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で確立した高分解能かつ高感度な酸化脂質の測定分析方法は、ヒトの肺細胞に対する環境濃度オゾンの影響を研究する有効な方法である。それを用いて、日常大気中で観測された平均濃度と同様の低レベルのオゾンによるモデル肺サーファクタントとヒト肺細胞の安定性と機能性への影響を検討できるようになった。これらの基礎知見を活かし、極低濃度のオゾンによる健康被害及びその防止策に重要な指針を提示できると考えている。
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