2019 Fiscal Year Final Research Report
Construction of a useful compound-producing platform directly from CO2 via green algae
| Project/Area Number |
19K21147
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| Project/Area Number (Other) |
18H06006 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0601:Agricultural chemistry and related fields
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| Research Institution | Tokyo University of Technology |
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Principal Investigator |
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | クラミドモナス形質転換体の創出 / クラミドモナスの効率的な光培養系の構築 / クラミドモナス用の形質転換系の新規構築 / グリセロール資化性酵母の単離 / 各種分析機器を用いた代謝物評価系の構築の完了 |
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| Outline of Final Research Achievements |
Metabolic modification of C. reinhardtii was tried to construct glycerol-producing green algal strain. After creation of pChlamy_4-GPP with expression vector pChlamy_4 and S. cerevisiae GPP, C. reinhardtii/pChlamy_4-GPP was constructed by using transformation system with NEPA-Gene to enhance the transformation efficiency. To evaluate glycerol production, its evaluating system with HPLC was set up, and the extraction process of glycerol from C. reinhardtii is also being improved. Aspect of culturing system, after scaling up photo bioreactor for C. reinhardtii to 3 L, stable culturing was demonstrated with culture optimization. Regarding glycerol assimilating yeast, no. 7 strain was selected from S. cerevisiae cultured in medium including glycerol as a sole carbon source. The ethanol evaluating system was also constructed with GC.
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| Free Research Field |
農芸化学
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| Academic Significance and Societal Importance of the Research Achievements |
クラミドモナスの形質転換体の構築に関し、既存の形質転換法では形質転換体が得られなかったため、NEPA-Geneを用いた形質転換系の最適化を図った。また出芽酵母由来のグリセロール資化性酵母を単離した。共培養系だけではなく、今後グリセロール資化性酵母の培養条件を最適化するとともに資化性能を向上させ、産業副産物として生産されるグリセロールの使用用途を拡充できる可能性がある。またこの度実験系として考案し研究を進めた緑藻-グリセロール資化性酵母の共培養系は、以後のCO2から直接的に物質生産できる可能性を有するという点で有意義であったと考えた。
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