2019 Fiscal Year Final Research Report
Optimization of base editing technology
| Project/Area Number |
19K21180
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| Project/Area Number (Other) |
18H06050 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0701:Biology at molecular to cellular levels, and related fields
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| Research Institution | Kobe University |
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Principal Investigator |
Yoshioka Shin 神戸大学, 科学技術イノベーション研究科, 特命助教 (50821980)
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | Base editing / ゲノム編集 |
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| Outline of Final Research Achievements |
Base editing such as Target-AID is a new genome editing technology that enable direct DNA base conversion without DNA double strand break. This technology has advantages such as lower cytotoxicity and easier introduction of targeted base replacement compare to the conventional genome editing tools.
We found that the CDA1 attached to the N terminal of nCas9 increased genome editing efficiency. Also, to shorten or lengthen the sgRNA shifted editing window.
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| Free Research Field |
ゲノム編集
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| Academic Significance and Societal Importance of the Research Achievements |
塩基編集技術はDNA二本鎖切断を介さずに直接ゲノムを書き換えることが可能であることから、DNA二本鎖切断を前提とする一般的なCRISPR/Cas9 systemと比較してより安全におよび正確にゲノム編集を行うことができる。これらのことから医療などへの応用展開が期待される重要なゲノム改変技術である。その為、本研究を通して得られた基盤技術であるTarget-AIDの特性は今後の応用へ向けた技術開発に重要なものとなる。
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