2019 Fiscal Year Final Research Report
Rational design of aptamer-tagged tRNAs
| Project/Area Number |
19K21181
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| Project/Area Number (Other) |
18H06055 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0701:Biology at molecular to cellular levels, and related fields
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| Research Institution | Rikkyo University |
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Principal Investigator |
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | tRNA / アプタマー / 遺伝暗号 |
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| Outline of Final Research Achievements |
In this study, tRNA variants tagged with a small hairpin RNA aptamer were developed without compromising the translation activities of the tRNAs and the target binding activities of the aptamers. A novel group of tRNAs (allo-tRNAs) was used as tRNA chassis. The allo-tRNA variants transferred at least four kinds of amino acids (serine, alanine, tyrosine, and histidine) into the ribosome in E. coli. Upon overexpression of the target proteins for transplanted aptamers, the allo-tRNA variants were sequestered from the translation apparatus of the cell. Aptamer-tagged tRNAs may be useful for radical and dynamic manipulation of the genetic code.
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| Free Research Field |
合成生物学
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| Academic Significance and Societal Importance of the Research Achievements |
遺伝暗号システムのダイナミックな改変や制御を行うための基盤的技術を発明した。アミノ酸を運搬するtRNA分子の改変自由度が飛躍的に向上し、将来的には様々な人工のアミノ酸や核酸塩基を用いたタンパク質工学やRNA工学の実現が期待される。無細胞タンパク質合成系の性能向上にも役立つ。更に、細胞内のtRNA分子集団の機能をON/OFF制御できるため、細胞内における２つの遺伝暗号システムの切り替えが実現しうる。
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