Search for new bioactive compounds utilizing the induction of secondary metabolite production by co-culturing fungi

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21227
• Project/Area Number (Other): 18H06109 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0801:Pharmaceutical sciences and related fields
• Research Institution: Kumamoto University
• Principal Investigator: Eguchi Keisuke 熊本大学, 大学院生命科学研究部(薬), 特別研究員 (60822799)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 二次代謝産物 / 真菌 / 医薬品シーズ / 共培養

Outline of Final Research Achievements

The discolored area was analyzed by LC-MS for the combination of the fungal strains 17F4103 and 17F4110 isolated from soil in Hyuga City, Miyazaki Prefecture. As a result, the presence of a compound (m/z 371 [M-H]-) that could not be confirmed by mono-culture was confirmed. Therefore, large-scale culture was performed for the purpose to afford these compounds, and the MeOH extract of the culture was purified to afford Penicidone D (1) and vermixocin A (2). The target compound of m/z 371 [M-H]- was identified as a known vermixocin A (2). Accordingly, this research found that co-culture experiment has drawn the structure diversitiy of natural product.

Free Research Field

天然物化学

Academic Significance and Societal Importance of the Research Achievements

これまでに微生物からの医薬シーズ探索が世界中で精力的に行われた結果、新しい骨格や生物活性を有する化合物の発見が次第に困難になってきているのが現状である。したがって、従来とは異なる方法で微生物資源からの医薬シーズ探索に挑戦することにより、新規性の高い化合物が発見されることが期待できる。そこで共培養を用いることでコンビナトリアル合成などの有機合成化学的手法では取得できない化合物の取得を目指し、新規骨格を有する医薬シーズを発見することで創薬研究に貢献できると考えている。