2019 Fiscal Year Final Research Report
Elucidation of the mechanisms for Sansoninto to produce the brain-derived neurotrophic factor
| Project/Area Number |
19K21237
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| Project/Area Number (Other) |
18H06119 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0801:Pharmaceutical sciences and related fields
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| Research Institution | Matsuyama University |
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Principal Investigator |
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | 酸棗仁湯 / 脳由来神経栄養因子 / 育薬 / 海馬 |
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| Outline of Final Research Achievements |
In this study, we examined the mechanisms of Sansoninto promoting the production of brain-derived neurotrophic factor (BDNF). When the five constituent herbal extracts were added to Neuro2a cells, the phosphorylation level of protein kinase A (PKA) and cAMP response element binding protein (CREB) were enhanced by jujube seed extraction. Moreover, the protein expression of matureBDNF was also increased. Similar to this results, BDNF production via PKA signal activation was confirmed in mouse hippocampal tissue. These results suggest that the activation of PKA signal by jujube seed contributes to the promotion of BDNF production by Sansoninto.
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| Free Research Field |
薬理学
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| Academic Significance and Societal Importance of the Research Achievements |
認知症やうつ病などの中枢神経疾患では脳内でのBDNF発現が低下する．報告者はこれまでに，不眠症改善に用いられる漢方方剤「酸棗仁湯」がマウス脳内においてBDNF産生促進作用を発揮することが明らかにしたが，詳細なメカニズムについては不明であった．本研究では，酸棗仁湯の構成生薬（サンソウニン，ブクリョウ，チモ，センキュウ，カンゾウ）のうち，サンソウニン抽出エキスが細胞培養系およびマウス海馬においてPKAシグナル活性化し，BDNF発現を上昇させること見出した．これらの研究成果は，酸棗仁湯を中枢神経疾患治療薬として臨床応用するためのエビデンス構築に寄与するものである．
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