Clarification of molecular bases for signal transduction by "signaling endosomes"

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21238
• Project/Area Number (Other): 18H06120 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0802:Biomedical structure and function and related fields
• Research Institution: Tohoku University
• Principal Investigator: Tsutsumi Ryouhei 東北大学, 薬学研究科, 助教 (50435872)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: エンドサイトーシス / 細胞増殖因子 / 細胞内情報伝達

Outline of Final Research Achievements

Recently, the "signaling endosome (SE) model" has been proposed in which cell surface receptors evoke signaling not from the plasma membrane, but from endocytic vesicles. This project aimed to clarify molecular bases of such SEs.
Outputs during this term of grant support are; optimization of a method to isolate SEs using magnetic nano-particles, identification of many signaling- and metabolism-associated proteins at SEs, and development of a method to efficiently detect protein sulfenylation and perthiosulfenylation.
Results from this project including above were presented at domestic and international conferences and were published from scientific journals.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

細胞は外部からの刺激に応じて細胞内情報伝達を惹起し、増殖や分化、運動など様々な応答を示す。この情報伝達の以上はがんなどの様々な疾病の原因となるが、一方でその分子機構は必ずしも明らかにされていない。本研究では、従来の細胞膜からの情報伝達というモデルは十分ではないとする「シグナリングエンドソーム」モデルの解明を目指し、その詳細を検討した。得られた成果は上記のような疾患の原因を解明するうえで重要な知見となることが期待される。