Cell-type specific calcium imaging of suprachiasmatic nucleus with fiber photometry

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21241
• Project/Area Number (Other): 18H06123 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0802:Biomedical structure and function and related fields
• Research Institution: Kanazawa University
• Principal Investigator: TSUNO Yusuke 金沢大学, 医学系, 助教 (70827154)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: サーカディアンリズム / 視交叉上核 / ファイバーフォトメトリー / カルシウムイメージング / インビボ

Outline of Final Research Achievements

Using fiber photometry, we found that the relationship between calcium rhythms of AVP neurons and animal behavioral rhythms was disrupted in circadian rhythm-deficient mice in which suprachiasmatic nucleus AVP neurons could not release GABA specifically. (Maejima, Tsuno et al., Submitted). Long-term recordings of suprachiasmatic nucleus AVP neurons in freely moving mice showed that the peak of calcium rhythm usually corresponds to the end of animal behavior, but that the relationship was broken in knockout mice.

Free Research Field

神経生理学

Academic Significance and Societal Importance of the Research Achievements

視交叉上核ニューロンの中でも、AVPニューロンの重要性を示唆する結果であり、本研究は学術的に意義深い。サーカディアンリズムは、昼の活動と、夜の睡眠のリズムを制御し、この変調は睡眠障害や精神疾患の原因となりうる。サーカディアンリズムの生成メカニズムを理解することで、睡眠障害やシフト勤務といった日内リズムの変調を正す手掛かりが得られる。