Analysis of intestinal absorption of botulinum toxin complex type B

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21257
• Project/Area Number (Other): 18H06142 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Kanazawa University
• Principal Investigator: Amatsu Sho 金沢大学, 医学系, 助教 (90827346)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: ボツリヌス毒素 / ヘマグルチニン / 腸管吸収 / トランスサイトーシス

Outline of Final Research Achievements

I studied the interaction between botulinum toxin complex type B and the candidate proteins which were identified as transcytosis receptor in intestinal epithelia. In in vitro experiments, Caco-2 cell and CMT-93 cell were used as a intestinal absorption model. In these cells grown on a transwell filter, the candidate protein is co-localized with botulinum toxin complex endocytosed from apical membrane of the cell. Caco-2 cell and CMT-93 cell were transfected with a CRISPR-Cas9 plasmid. After clone isolation, I will analyze the endocytosis and transcytosis efficiency of the cells lacking the candidate protein.

Free Research Field

細菌毒素

Academic Significance and Societal Importance of the Research Achievements

ボツリヌス毒素複合体は食中毒の原因因子であり、致死率の高い毒素として知られている。経口摂取された毒素は腸上皮から吸収される。本毒素の腸管吸収機構を理解することで、ボツリヌス症の病態発症メカニズムを解明することができる。また、発症メカニズムを基にしたボツリヌス症に対する新たな治療法の開発へ繋がることが期待できる。