2020 Fiscal Year Final Research Report
Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration
Project/Area Number |
19K21307
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Project/Area Number (Other) |
18H06203 (2018)
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund (2019) Single-year Grants (2018) |
Review Section |
0902:General internal medicine and related fields
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Research Institution | Jikei University School of Medicine |
Principal Investigator |
SUSUMU TAJIRI 東京慈恵会医科大学, 医学部, 助教 (50646362)
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Project Period (FY) |
2018-08-24 – 2021-03-31
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Keywords | 慢性腎不全 / 腎臓再生 / 多能性幹細胞 / ネフロン前駆細胞 |
Outline of Final Research Achievements |
It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells affecting their differentiation and angiogenic potential. Recent studies reported that specific abnormalities caused by the non-inherited disease are often retained in induced pluripotent stem cell(iPSC)-derived products obtained from patients. Thus, it is indispensable to assess whether iPSCs derived from patients with CKD due to non-inherited disease have the ability to generate kidneys. We generated iPSCs from patients undergoing haemodialysis due to non-inherited disease (HD-iPSCs) or from healthy controls (HC-iPSCs). HD-iPSCs differentiated into nephron progenitor cells (NPCs) with similar efficiency to HC-iPSCs. Additionally, HD-iPSC-derived NPCs expressed comparable levels of NPC markers and differentiated into vascularised glomeruli upon transplantation into mice, as HC-iPSC-derived NPCs. Our results indicate the potential of HD-iPSCs as a feasible cell source for kidney regeneration.
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Free Research Field |
再生医学
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Academic Significance and Societal Importance of the Research Achievements |
慢性腎不全に対する根本的治療としては腎移植があるが、ドナー不足の問題から腎移植のみでは腎不全を克服することは難しい。また仮に腎移植をうけられた場合も生涯にわたり、免疫抑制剤の内服が必要になる。患者由来の多能性幹細胞から腎臓を再生し、移植することができれば、ドナー不足や免疫抑制剤の内服といった問題を克服することができる。本研究は、患者由来幹細胞由来の腎臓再生の可能性を示すものである。
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