2019 Fiscal Year Final Research Report
Study on multifocal human papillomavirus infection in tonsils
| Project/Area Number |
19K21350
|
|---|
| Project/Area Number (Other) |
18H06256 (2018)
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
|---|
| Review Section |
0906:Surgery related to the biological and sensory functions and related fields
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2018-08-24 – 2020-03-31
|
| Keywords | ヒトパピローマウイルス（HPV) / 扁桃 / PCR / p16 |
|---|
| Outline of Final Research Achievements |
We tried to detect HPV DNA using auto-nested PCR to investigate HPV infection in tonsillar tissues other than affected tonsils in human papillomavirus (HPV) -related oropharyngeal cancer, but it was difficult to detect. We are considering to perform PCR by different methods. To examine HPV infection in the isolated palatine tonsils of non-cancer patients, p16 immunostaining, a surrogate marker for HPV infection, was performed in 79 cases because PCR was difficult, and all were negative. Once the PCR detection method is established, PCR will be performed on the p16 immunostained sample for comparison.
|
| Free Research Field |
耳鼻咽喉科学
|
| Academic Significance and Societal Importance of the Research Achievements |
HPV 関連中咽頭癌の非癌扁桃組織におけるHPV 感染率を知ることで、HPV がmultifocal に感染し第二のプライマリー、第三のプライマリーを生じる可能性を考察することができ、今後のHPV関連中咽頭癌の治療戦略の上で非常に重要なこととなりえる。また、非癌患者の摘出口蓋扁桃におけるHPV 感染を知ることで口蓋扁桃へのHPV 感染の年齢層別の特徴を見いだすことや、患者背景因子や嗜好歴などを比較することができ、口蓋扁桃へのHPV 感染のリスク因子を考察することができる。さらにp16 免疫染色と対比することにより、oncogenic なHPV 感染が生じ得るのかも検討できる。
|
