2019 Fiscal Year Final Research Report
Elucidation of bone marrow niche signal using epigenome analysis and establishment of bone marrow reconstitution culture method
| Project/Area Number |
19K21388
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| Project/Area Number (Other) |
18H06300 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | The University of Tokyo (2019)
Tsurumi University (2018) |
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Principal Investigator |
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | 骨髄 / 多能性前駆細胞 / 幹細胞niche / single cell RNA-seq / RNA-seq / ATAC-seq |
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| Outline of Final Research Achievements |
The author examined to isolate mesenchymal cell populations by analyzing MSCs at the single cell level. The MSCs were analyzed by scRNA-Seq, and seven specific clusters were confirmed from the gene expression profile. In addition, each cluster were confirmed that they could be separated using FACS from the gene expression profile. Furthermore, author revealed that seven clusters were independent cell populations by RNA-Seq and ATAC-seq analysis. Next, author performed in vitro characterization of these clusters. As a result, aouthor found that some of the clusters had a function closely stem cells. Author aim to identify stem cell-like cell populations by direct comparison with ES cells in the future.
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| Free Research Field |
再生医学
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| Academic Significance and Societal Importance of the Research Achievements |
組織幹細胞はヘテロな細胞集団であるため、本研究では、先行報告されている骨髄幹細胞マーカーで分離した細胞群には7つの細胞集団が存在することを明らかにし、それぞれが独立した細胞集団であることを証明した。これらのクラスターのうち幹細胞特性に極めて類似したクラスターを同定するため、バイオインフォマティックス解析を行った。結果として、幹細胞類似集団の同定には至らなかったが、特性解析から分化度が低く、分化指向性を有する細胞集団を特定することができたことから、必要な組織への分化誘導が可能なり、また培養することにより必要量を確保できることから、臨床への期待値は大きいと考える。
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