RANKL expression in response to mechanical stress in osteocyte

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21405
• Project/Area Number (Other): 18H06321 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0907:Oral science and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Shoji Ayumi 東京医科歯科大学, 歯学部附属病院, 医員 (60826254)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 歯の移動 / 骨細胞 / RANKL

Outline of Final Research Achievements

Osteocytes, the most numerous cells in bone, are stellate-shaped cells enclosed within a bone lacunocanalicular network and have been shown to function as mechanosensory cells in bone. Osteocyte-derived RANKL plays a crucial role in the regulation of osteoclastogenesis during orthodontic tooth movement. Although osteocytes have been thought to be the functional regulator in orthodontic tooth movement, it is not well-understood how they contribute to the alveolar bone remodeling via orthodontic force. In this study, I established a roading method to add mechanical stress (Fluid flow sheer stress; FFSS) on MLO-Y4, continue to confirm RANKL, OPG, Opn expression by using RT-PCR.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

本研究での矯正力(=メカニカルストレス)を感受・応答する骨リモデリング分子の同定とその制御機構の解明は、 骨の動的な恒常性とその破綻により発症する疾患を新たな枠組みで理解することを可能にする。マウス骨細胞様細胞株(MLO-Y4)へのメカニカルストレス （流体剪断応力；FFSS）付与の実験系の構築により、今後の矯正歯科治療における新たな治療法開発の分子的基盤の確立につながることが期待される。