Single-cell analysis using a 3D-integrated nanopore

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22108
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 28:Nano/micro science and related fields
• Research Institution: Osaka University
• Principal Investigator: Tsutsui Makusu 大阪大学, 産業科学研究所, 准教授 (50546596)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: ナノポア / 電気穿孔 / 1細胞解析 / 電気泳動 / イオン電流

Outline of Final Research Achievements

A 3D-integrated nanopore was fabricated that consisted of a multipore membrane stacked vertically on a SiNx nanopore membrane via a thick polyimide layer. This novel nanosensor was used to measure intra-cellular molecules of E. coli. When the bacterial cell was trapped at the multipore, its membrane surface was ruptured by the strong electric field there that enabled extraction to resistive pulse sensing of the molecules inside including ribosomes and DNA.

Free Research Field

マイクロ・ナノ科学

Academic Significance and Societal Importance of the Research Achievements

本研究で創成した積層集積ナノポアによる1細胞内物質分析法は、細孔サイズ如何でDNAから細胞小器官まであらゆる細胞内粒子・分子の解析が可能な原理を有する。今後、当該技術が医療分野などで広く応用されることで、ハイスループット且つ超高感度な細胞診が実現でき、早期がん診断、ひいては遺伝子レベルの治療法の可能性を拓くことができると期待される。