Multi-scale holographic stimulation technique for higher-level optogenetics

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22146
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 30:Applied physics and engineering and related fields
• Research Institution: Kobe University
• Principal Investigator: Matoba Osamu 神戸大学, 先端融合研究環, 教授 (20282593)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: ホログラフィック光刺激 / オプトジェネティクス

Outline of Final Research Achievements

In this research, we developed the holographic stimulation technique for achieving a higher-level optogenetics, that can control cell activity by optical irradiation. In the developed technique, multiple spots are generated in a three-dimensional space and beam spot size can be changed from 1 um to 20 um. Furthermore, uniform peak intensity of multiple spots can be created regardless of a number of spots. The developed technique was applied to cultured cells and selective control of cells was confirmed experimentally.

Free Research Field

応用光学

Academic Significance and Societal Importance of the Research Achievements

生命機能情報の非接触，低侵襲観察を元にした生命機能の解明や細胞活動の非接触操作により生命機能を改善するなど，生命科学における光イメージング技術及び光照明技術に対する期待は大きい。本研究の研究成果は所望の３次元位置に多数の光スポットを同時形成することを可能にし，さらに高性能な光スポット群を形成する技術を開拓したものであり，この技術を発展させることで生命科学における有用な光操作技術の確立に繋がる。