2022 Fiscal Year Final Research Report

Development of a multi-step salt catalyst pressurized microwave treatment system for complete energy conversion of holocellulose

Research Project

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Project/Area Number	19K22155
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 31:Nuclear engineering, earth resources engineering, energy engineering, and related fields
Research Institution	The University of Tokushima
Principal Investigator	ASADA Chikako 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (10580954)
Co-Investigator(Kenkyū-buntansha)	中村嘉利徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (20172455) 阪本鷹行徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 講師 (90740332)
Project Period (FY)	2019-06-28 – 2023-03-31
Keywords	バイオエタノール
Outline of Final Research Achievements	To utilize cellulose contained in un-utilized sugarcane bagasse as a substrate for ethanol production, an ecotype system, i.e. pressurized microwave hydrothermal treatment with inorganic salts, and simultaneous saccharification and fermentation (SSF) were studied using salt-tolerant cellulase, i.e. Meicelase, and salt-tolerant yeast, i.e. Saccharomyces cerevisiae BA11. The component analysis, enzymatic saccharification, and alcohol fermentation were carried out using pressurized microwave treated sugarcane bagasse with various inorganic salts. The pressurized microwave treatment using 2 wt% MgCl2 at a treatment temperature of 200oC for a treatment time of 5 min followed by SSF without washing treatment of inorganic salts provided the maximum glucose and ethanol yields, i.e. 0.392 and 0.18 g/g-raw material, those corresponded to 99 and 90 % of saccharification and ethanol conversion ratios, respectively.
Free Research Field	生物化学工学
Academic Significance and Societal Importance of the Research Achievements	既往のバイオマス利用には、収率、設備、廃液処理やランニングコスト等さまざまな問題がある。応募者らも水蒸気爆砕等を用いた木質バイオマスの処理を行ってきたが超高温高圧条件でないと前処理効果が小さいことやアルコール発酵阻害物（フルフラールや5-HMF）の大量発生などの問題点があった。バイオマスはこれまで、ペレット燃料かセルロース成分のみに注目されていたが、本提案システムによりすべての原料構成成分を有効に利用できることになる。