Single Molecule Analysis of Modified Nucleotides Using Quantum DNA Sequencer

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22244
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 37:Biomolecular chemistry and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: SANDO SHINSUKE 東京大学, 大学院工学系研究科(工学部), 教授 (20346084)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 量子シークエンサー / DNA / 配列解析 / 1分子計測

Outline of Final Research Achievements

1.5-BzIm, dU derivative having benzimidazole structure at 5-position, was quantitatively formed by the oxidative cyclization of the modified nucleobase, 5-formyl-dU (5-fdU), with o-phenylenediamine.　2.5-BzIm has a HOMO orbital close to the Fermi level of gold, resulting in a higher tunneling current than dT, 5-fdU, and dG.　3.We also focused on the interaction between the gold electrode and the functional groups; among the modified dUs with ethynyl, cyano, and amino groups at the 5-position, EtdU with ethynyl group exhibited a larger tunneling current. 　4.It was confirmed that the base calling accuracy of EtdU can be improved by analysis using the two variables, the magnitude of the tunneling current and CV value.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

量子シークエンサーは、核酸の配列解析を可能にする次々世代シークエンサーとして、その実現が期待されています。本研究成果は、検出が困難と考えられてきた「修飾を受けた塩基」に対する解析に向けた方法を提案しています。