Development of A Super-Sensitive Immunolabeling Method Utilizing DNA Origami Molecular Machines

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22261
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 37:Biomolecular chemistry and related fields
• Research Institution: Kansai University
• Principal Investigator: Kuzuya Akinori 関西大学, 化学生命工学部, 教授 (00456154)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: DNAオリガミ / 人工抗体

Outline of Final Research Achievements

A "substrate recognition site" to recognize practical targets used in immunostaining and ELISA and a "signal transmission site" to detect structural changes as fluorescence and enzymatic activity were incorporated to Project Leader's original "DNA Origami molecular machine", to develop an "allosteric artificial antibody" that is unprecedented in the world. By employing novel operating mechanism of the DNA Origami molecular machine, selective on/off control of signal transmission was successfully accomplished.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

今日までにがんの治療薬として「抗体医薬」の有用性が広く認知されてきたが、タンパク質である抗体分子の製造には大きなコストがかかるため、これらの普及は同時に、我が国の今後の医療費の高騰を引き起こす可能性があることが指摘されてきた。本研究が目的とする核酸ベースの人工抗体を実用化することができれば、抗体医薬の抗原認識部位だけを取り出して活用することで、非常に安価な後発医薬品を広範に提供できるプラットフォームを確立できる。