2020 Fiscal Year Final Research Report
A challenge to homochirality in glucose catabolism----evolutional analysis in L-glucose catabolism
| Project/Area Number |
19K22267
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2019-06-28 – 2021-03-31
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| Keywords | L-glucose catabolism / Rhizobiales / Verrucomicrobia / Luteolibacter / L-galactose |
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| Outline of Final Research Achievements |
In this study we first analyzed the ability to utilize L-glucose in the type strains in the order Rhizobiales. Among 148 strains assayed, only Mesorhizobium acaciae JCM 30534T could utilize L-glucose, suggesting that the L-glucose utilization is not a common trait in this taxon.
Next, we analyzed the L-glucose-catabolic pathway in Luteolibacter sp. LG18, which belongs to different taxon at the phylum level from the known L-glucose utilizers. We identified a gene cluster of six genes in the LG18 genome, whose enzyme activities resemble in those of the L-glucose catabolic pathway in P. laeviglucosivorans 43P. However, precise enzymatic analyses of these genes suggested that the pathway in strain LG18 is different from that of strain 43P in part, including a C-4 epimerization, the enzyme of which is encoded by a gene outside of the cluster. Also, this gene cluster may have a function to utilize L-galactose.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
地球上のほとんどの生物はD-グルコースを資化することができるが、その鏡像体のL-グルコースを資化する生物は、長い間いないとされてきた。研究代表者は以前に自然界からL-グルコース資化菌P. laeviglucosivorans 43P株を単離し、その代謝機構を明らかにした。
本研究では、43P株とは分類学上の門レベルで異なるLuteolibacter LG18株のL-グルコース代謝機構を解析したものであり、43P株とは部分的に異なる代謝機構を有していることを示唆する結果を得た。これらの結果は、生物の糖代謝、特に希少糖代謝に関して学術的に重要な知見を与えるものである。
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