A study on innovative storage technology by using protein function which supports plant survival under the ultra-low temperature

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22274
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Shizuoka University
• Principal Investigator: Hara Masakazu 静岡大学, グリーン科学技術研究所, 教授 (10293614)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 超低温保存 / タンパク質保護 / 天然変性タンパク質 / デハイドリン

Outline of Final Research Achievements

Recently, biopharmaceuticals especially antibody drugs have been actively developed. A large amount of preservatives and cold storage are required to stabilize the antibody solution. Freezing and thawing, however, promotes the antibody aggregation, and then the drug performance is remarkably decreased. Meanwhile, we found that F segment of dehydrin, a protein involved in the freezing stability of plant seeds, potently inhibited the cryodenaturation of proteins. In this study, we showed that the cryoaggregation of antibody could be efficiently inhibited by F segment, discussing the mechanism of cryoprotection.

Free Research Field

植物機能生理学

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、種子の低温耐性に関与し、一定の形をとらない天然変性タンパク質であるデハイドリンが、他のタンパク質を凍結ストレスから守る仕組みを解明した点である。社会的意義としては、デハイドリンの機能がバイオ医薬品、特に抗体製剤の保存技術を格段に向上させる可能性を示した点である。