2021 Fiscal Year Final Research Report
Molecular mechanism of high vitamin C accumulation in fruits
| Project/Area Number |
19K22284
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸田 隆典 島根大学, 学術研究院農生命科学系, 教授 (50607439)
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| Project Period (FY) |
2019-06-28 – 2022-03-31
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| Keywords | アスコルビン酸 / 果実 / ガラクツロン酸経路 / 遺伝子発現 |
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| Outline of Final Research Achievements |
In general, plant fruits contain large amounts of ascorbic acid, with concentrations reaching as high as 10% of total soluble carbohydrates, and are an important source of vitamin C for us humans. However, the mechanism and physiological significance of why ascorbic acid accumulates in high concentrations in fruits is a completely unexplored question. Based on our previous study that the ASC biosynthetic pathway is induced and activated via D-galacturonic acid (D-GalUA), which has not yet been determined in mature fruit, we proceeded to analyze tomato D-GalUA reductase. The results of this study suggest a strong possibility that SIAKR1 is a novel enzyme protein involved in D-GalUA reduction activity.
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| Free Research Field |
応用生物化学
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| Academic Significance and Societal Importance of the Research Achievements |
果実にビタミンCが豊富に含まれていることは一般によく知られていることであるが、なぜ、どのようにして蓄積しているのか？その機構は良くわかっていない。本研究では、植物で遺伝的に未確定のD-GalUA経路の関与を実験的に予測し、その代謝に関わる酵素としてD-GalUA還元酵素の候補遺伝子（SIAKR）を得た。本成果は学術的に新規の知見であるとともに、今後SIAKRの機能解析を進めることで、上記の謎を解き明かすととともに、社会的には高ビタミンC果実の育種に繋がる可能性を秘めている。
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