Development and application of in vitro culture of OsHV-1, an important viral pathogen for oyster culture

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22325
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: Itoh Naoki 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (30502736)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: マガキ / ウイルス / 組織培養 / 細胞培養

Outline of Final Research Achievements

The aim of this project was to establish a proliferation system of OsHV-1, an significantly important viral pathogen for oyster culture, using in vitro cultured oyster cells and organs. Amount of OsHV-1 was found to increase when the viral suspension was added to primary cultured oyster hemocytes from Day 3 to Day 7, but the increase was not significant compared to the control group, suggesting that hemocyte cell was not the target cell type for OsHV-1. Then, viral suspension was added into gills, mantle or heart organs maintained in a culture medium. OsHV-1 DNA was not detected in mantle and heart, but consistently detected in gill tissues, indicating that gill is a suitable tissue to proliferate OsHV-1 in vitro. However, OsHV-1 was not recovered from the used culture medium in the experiment, and further improvement is required to apply this method to obtain large amount of pure OsHV-1.

Free Research Field

魚病学

Academic Significance and Societal Importance of the Research Achievements

当初はマガキの血球を用いマガキの病原ウイルスであるOsHV-Iを安定的に増殖させることを目指したが、達成には至らなかった。一方、血球に代えて培養鰓組織片を用いると、OsHV-Iをin vitroで人為的に増殖させることができる可能性が初めて見出された。鰓組織は採取が容易かつ多量に取得可能であるため、今後、手法を改良することで、OsHV-I研究の進展が期待できる。