Identification of novel molecules on lysosomal membrane using our unique technique

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22381
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Hasegawa Junya 東京医科歯科大学, 難治疾患研究所, 助教 (00533788)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: イノシトールリン脂質 / リソソーム / 質量分析

Outline of Final Research Achievements

Lysosomes are acidic organelles, which are crucial for the degradation of many different proteins and lipids. The degradative products (e.g. amino acids) are then released and reused for cell survival. However, it has been unclear what proteins/lipids are included on the membrane of lysosomes. We have established the method for the isolation of pure lysosomes. Using this method, we found that some interested phospholipids are detected in the lysosomal fraction, but not other fractions, by our LC-MS system.

Free Research Field

脂質生物学

Academic Significance and Societal Importance of the Research Achievements

リソソームの機能異常は、がんや中枢神経障害を含む様々な疾患の原因となる。リソソームの基盤的研究を推進し、その分子メカニズムを解明することは、上記疾患における創薬ターゲットの創出に貢献でき、各種疾患の予防、診断、治療法に繋がることが強く期待できる。