Genetic study to validate whether fairy chemicals are new plant hormone

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22412
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Utsunomiya University
• Principal Investigator: Okamoto Masanori 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (50455333)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 植物ホルモン / 成長制御 / 遺伝学 / 受容体 / 変異株

Outline of Final Research Achievements

The fairy molecules isolated from mushrooms as allelochemicals that control plant growth, are also present in a trace amount in plants and have properties that fit the definition of new plant hormones. However, signaling components to induce physiological effects via fairy molecules have not yet been reported. Therefore, to elucidate the molecular action of fairy molecules, we aimed to isolate fairy molecule insensitive mutants using Arabidopsis thaliana, an excellent model plant for molecular biological studies. The E184 accession among Arabidopsis wild type accessions showed hypersensitive to the AHX. We performed EMS mutation treatment to the E184 accession and finally succeeded in isolating several mutant lines that are insensitive to AHX.

Free Research Field

植物分子生理学および農芸化学

Academic Significance and Societal Importance of the Research Achievements

これまでフェアリー分子群に対して感受性の変化した変異株の報告はない。得られた変異株の原因遺伝子を明らかにすることで、フェアリー分子の作用秩序の分子機構が進むことが期待される。フェアリー分子のシグナル伝達因子が明らかになることで、それら遺伝子を操作することで、植物分子育種の研究へと発展する。さらに、シグナル伝達因子に作用する化合物探索の研究は、新しい植物成長調節剤の開発に発展する事が期待される。