Development of a pollen-mediated gene transfer method

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22432
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Yoshida Satoko 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (20450421)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 遺伝子導入 / 花粉 / マグネトフェクション

Outline of Final Research Achievements

In this study, we investigate a pollen-mediated gene transfer method. Conventional methods of gene transfer, such as Agrobacterium-mediated methods and methods using particle guns, need to regenerate plants from callus or cells to establish the transgene-containing individuals. However, the regeneration condition differ depending on plant cultivars, and it takes an enormous amount of time to find the optimal regeneration method. In this study, targeting pollen as the germplasm, gene transfer via Agrobacterium and magnetofection were investigated. Although incorporation into the genome was not confirmed, introduction of DNA into pollen by magnetofection was confirmed.

Free Research Field

植物分子生物学

Academic Significance and Societal Importance of the Research Achievements

増え続ける世界人口や環境変動による農作地の喪失による食料危機に対応するためには、農作物の改変による食料の増産が必要不可欠である。近年、ゲノム解析技術やゲノム編集技術が大きく発展しているが、すべての植物に共通して利用できる、世代継承できる遺伝子導入技術は開発されていない。本研究では、生殖細胞である花粉をターゲットとして、遺伝子導入技術の開発を検討した。