2020 Fiscal Year Final Research Report
| Project/Area Number |
19K22532
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 49:Pathology, infection/immunology, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
Sato Shintaro 大阪大学, 微生物病研究所, 特任准教授(常勤) (80447333)
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| Project Period (FY) |
2019-06-28 – 2021-03-31
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| Keywords | ヒトノロウイルス / 受容体 |
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| Outline of Final Research Achievements |
In order to screen for human norovirus receptors using the CRISPR/Cas9 system, we searched for human norovirus lots that have a cytopathic effect, however, no cytopathic effect was observed even when the virus concentration was increased 100 times. Five genes that may be involved in human norovirus infection and proliferation were determined. Each was forcibly expressed using a lentiviral vector in epithelial cells showing no propagation of human norovirus. We are currently investigating the ability of these cells to proliferate human norovirus.
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| Free Research Field |
粘膜免疫学
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトノロウイルスのin vitro増殖が可能になってからすでに5年近く経過したが、その増殖系を用いたヒトノロウイルスの性状解析などは未だに進んでおらず、申請者らも挑戦しているが、細胞変性効果を示す実験系も確立されていない。今後も地道に解析を継続することで、ヒトノロウイルスに対するワクチン、治療薬の開発に繋がるものと思われる。
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