Dissecting transcription factors using proximity labeling analyses

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22607
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 52:General internal medicine and related fields
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Narumi Satoshi 国立研究開発法人国立成育医療研究センター, 分子内分泌研究部, 室長 (40365317)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 転写 / 転写制御 / プロキシミティラベリング / 転写因子 / 変異 / 転写複合体

Outline of Final Research Achievements

Transcriptional regulation is a molecular mechanism that controls the turning on and off of gene functions, but understanding of which molecules are involved in this regulation is lacking. In this study, we employed proximity labeling analysis, a new technique to discover molecular interactions, to analyze transcription factors involved in congenital diseases. As a result, we detected several previously unknown interactions in addition to the previously known ones. This study demonstrates the broad applicability of proximity labeling analysis in the study of transcriptional regulatory mechanisms.

Free Research Field

分子遺伝学

Academic Significance and Societal Importance of the Research Achievements

本研究は転写制御機構研究におけるプロキシミティラベリング法の応用可能性の広さを示すと同時に、本研究の独自性である「野生型分子と変異型分子を比較解析」が転写制御機構研究の有用な切り口になることを示した。現在普及している「転写促進複合体」「転写抑制複合体」という二項対立モデルは適切ではなく、実際には両者が柔軟・可塑的に使い分けられている可能性があり、今後のこの研究分野における研究の方向性を示す成果と言える。