High-throughput verification of pathways regulating kidney diseases

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22628
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 53:Organ-based internal medicine and related fields
• Research Institution: Tokai University
• Principal Investigator: MATSUSAKA Taiji 東海大学, 医学部, 教授 (50317749)
• Co-Investigator(Kenkyū-buntansha): 梅津 光央 東北大学, 工学研究科, 教授 (70333846)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 腎臓病 / 低分子抗体 / ポドサイト / ガイドRNA

Outline of Final Research Achievements

Gene expression pattern is dynamically changed in kidney diseases. To study gene functions in kidney diseases, we aimed to develop a simple method to modify a specific gene in a mouse body with kidney disease. We generated mice in which the kidney expresses a gene-modifying enzyme and tried to develop a carrier protein that delivers guide RNA to kidney cells. The carrier protein is taken up into kidney cells, however further improvement is necessary to accomplish this project.

Free Research Field

腎臓内科学

Academic Significance and Societal Importance of the Research Achievements

期間内に達成できなかったが、核酸を特定の細胞に送り込む技術は、医学生物学研究を加速させる基盤技術となるし、治療薬としての利用が注目されている核酸の腎臓への送達技術は、臨床的にも重要である。今研究で明らかになった技術的問題の解決が望まれる。