Regeneration for vocal fold sulcus with amniotic membrane

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22691
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Hirano Shigeru 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10303827)
• Co-Investigator(Kenkyū-buntansha): 椋代 茂之 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00821860) ; 杉山 庸一郎 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50629566)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 声帯再生 / 再生医療 / 声帯瘢痕 / 間葉系幹細胞 / 線維芽細胞 / 分化誘導 / 羊膜

Outline of Final Research Achievements

Vocal fold scar remains problematic. Particularly severe scar is still difficult to treat even with regenerative methods. We examined the effects of the amniotic membrane for regeneration of the whole vocal fold mucosa using rat and rabbits. Rat vocal fold fibroblasts were seeded on amniotic membrane in culture medium, and appropriate survival and proliferation were observed. Then the amniotic membrane was transplanted to the rabbit vocal folds. Regeneration of the vocal folds were observed at 1 month after the implant. The histology showed good deposition of hyaluronic acid in the lamina propria of the vocal folds. The results indicate the feasibility of the amniotic membrane for vocal fold regeneration.

Free Research Field

耳鼻咽喉科・頭頸部外科

Academic Significance and Societal Importance of the Research Achievements

重度の声帯瘢痕や声帯溝症に対する革新的な治療法の開発に向け、羊膜が科学的に有用であることを証明した。これらの疾患に苦しむ患者さんへの臨床応用が期待できる。