2020 Fiscal Year Final Research Report
The elucidation of transcriptional network controlling skeletal development by genome wide analysis
Project/Area Number |
19K22708
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 57:Oral science and related fields
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Research Institution | Osaka University |
Principal Investigator |
Hata Kenji 大阪大学, 歯学研究科, 准教授 (80444496)
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Project Period (FY) |
2019-06-28 – 2021-03-31
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Keywords | 軟骨細胞 / クロマチン構造 / ヒストン修飾 |
Outline of Final Research Achievements |
Chondrocytes play important roles during skeletal development. Accumulating evidence indicates that epigenetic regulation, such as histone modification and chromatin structure, is critical for gene expression. To understand the molecular mechanism of chondrocyte differentiation, we performed combination analysis of ChIP-seq and ATAC-seq using primary chondrocytes and identified chondrocyte specific enhancer regions. We have identified 31500 genomic regions as chondrocyte specific enhancer. Among them, we have identified chondrocyte specific enhancer for Sox9 and enhancer deletion mice showed the defects in endochondral bone formation. Moreover, peak-motif analysis identified several transcription factors involved in chondrocyte differentiation. Our findings would uncover novel transcriptional network controlling skeletal development.
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Free Research Field |
口腔生化学
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Academic Significance and Societal Importance of the Research Achievements |
本研究ではゲノム構造解析と遺伝子発現解析を統合的に行うことにより、DNAの塩基配列の変化ではなく、クロマチン構造の変化→ヒストン修飾→mRNA発現という階層的な遺伝子発現システムを体系的かつ網羅的にゲノムスケールで解明することが可能となった。生命計測技術の進歩と情報解析技術の進歩に伴って、様々な研究領域でゲノムワイド解析が応用され生命現象や疾患関連遺伝子の解明に貢献している。次世代シークエンサーを用いたゲノムワイド解析は今後の生命科学研究にとってますますその重要性を増すことが予測され、骨・軟骨研究における本研究の学術的意義は高い。
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