2020 Fiscal Year Final Research Report
Investigation of novel infection control strategy based on bacterial evolutional information
| Project/Area Number |
19K22710
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
住友 倫子 大阪大学, 歯学研究科, 講師 (50423421)
中田 匡宣 鹿児島大学, 医歯学域歯学系, 教授 (90444497)
広瀬 雄二郎 大阪大学, 歯学研究科, 特任研究員 (90788407)
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| Project Period (FY) |
2019-06-28 – 2021-03-31
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| Keywords | 肺炎球菌 / 化膿レンサ球菌 / 分子進化 / 低分子阻害剤 / ゲノムワイド関連解析 |
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| Outline of Final Research Achievements |
In this study, we attempted to establish novel strategy for identification of drug targets based on bacterial evolutionary analysis.
Our evolutionary analysis showed that among the examined pneumococcal genes, nanA and bgaA had high proportions of codon that were under significant negative selection. In addition, our in vitro and in vivo analyses indicated that BgaA works as a virulence factor via inducing vascular injury and blood coagulation.
Concerning Streptococcus pyogenes, our genome-wide association study using 351 genomes of S. pyogenes detected several genes and single nucleotide polymorphisms involved in the invasiveness.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
BgaAにおいて進化的に変異が制限されているコドンに着目したところ、タンパク質の構造を崩さない変異が選択されていることが示唆された。すなわち進化の保存性から酵素の機能に重要な部位が推定できる可能性が示された。
また化膿レンサ球菌において、病態と相関する遺伝的多様性が明らかになったことで、侵襲性感染症の予防や治療につながる礎となることが期待される。
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