2020 Fiscal Year Final Research Report

Fabrication of three-dimensional periodontal ligament tissue with nanometer-sized extracellular matrix films and creation of new system to analyze the cell-cell interaction

Research Project

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Project/Area Number	19K22711
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 57:Oral science and related fields
Research Institution	Osaka University
Principal Investigator	Takedachi Masahide 大阪大学, 歯学部附属病院, 講師 (60452447)
Co-Investigator(Kenkyū-buntansha)	北村正博大阪大学, 歯学研究科, 准教授 (10243247) 三木康史大阪大学, 歯学部附属病院, 助教 (10598395) 明石満大阪大学, 生命機能研究科, 特任教授(常勤) (20145460) 山口佳則大阪大学, 工学研究科, 招へい教授 (20386634)
Project Period (FY)	2019-06-28 – 2021-03-31
Keywords	歯根膜 / LbL法 / 細胞間相互作用
Outline of Final Research Achievements	We succeeded in fabrication of three-dimensional tissue consisting of human periodontal ligament cells (HPDL) and human vascular endothelial cells or human cementoblasts (HCEM) by using a Layer-by-Layer method. In addition, it has been clarified that three-dimensional culture of HPDL enhanced the expression of PLAP-1 and POSTN genes that are characteristically expressed in HPDL. On the other hand, analysis of cell-cell interactions revealed that co-culture of HPDL and HCEM induced the expression of the IBSP gene in HPDL and stimulated the ability of HPDL to differentiate to hard tissue-forming cells.
Free Research Field	歯周病学
Academic Significance and Societal Importance of the Research Achievements	ナノ薄層コーティング技術であるLbL法を世界で初めて歯科研究領域に取り入れ、歯周組織で最も重要な組織である歯根膜組織の三次元組織構築に成功したことは、今後、歯周病治療薬や歯周組織再生誘導剤の開発に際し、high throughputなスクリーニング方法の確立に向けた第一歩になった。また、細胞間相互作用の解明により新たな歯周組織恒常性維持機構の解明につながる情報が得られたものと考えられる。