• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2020 Fiscal Year Final Research Report

Introduction of proteolytic enzyme into biomineralization process and its application to tooth enamel regeneration

Research Project

  • PDF
Project/Area Number 19K22717
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionHiroshima University

Principal Investigator

Tanimoto Kotaro  広島大学, 医系科学研究科(歯), 教授 (20322240)

Co-Investigator(Kenkyū-buntansha) 國松 亮  広島大学, 病院(歯), 講師 (40580915)
加藤 功一  広島大学, 医系科学研究科(歯), 教授 (50283875)
廣瀬 尚人  広島大学, 医系科学研究科(歯), 助教 (50611935)
Project Period (FY) 2019-06-28 – 2021-03-31
Keywordsバイオミネラリゼーション
Outline of Final Research Achievements

We decided to use MMP-20 and KLK-4 to degrade residual proteins that are thought to be the cause of inhibition of HAP crystal induction using amelogenin, an enamel matrix protein, in order to verify whether crystal induction can be continued or not. The rh174 that constitutes the nanosphere has multiple recognition sites of MMP-20, and large and small fragments are formed by the degradation. Among them, the fragment containing the undegraded C-terminal domain remains on the growing HAP surface, but it was clarified by this experiment that further degradation occurs by further treatment with KLK-4.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

初期う蝕や軽度のエナメル質形成不全に対して、生体のエナメル質形成過程を模した人為的なバイオミネラリゼーションを生じさせることにより、修復治療を達成することさせることが本研究の最終目標である。本研究では、持続的な結晶成長の阻害となる結晶表面の残存蛋白を分解酵素により除去し、新たな結晶誘導を生じさせるための分解条件を明らかにした。完全分解には2種類の酵素が必要であることが示されたことに学術的意義があると思われる。

URL: 

Published: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi