Investigation for exercise-induced histone turnover in skeletal muscle

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22824
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
• Research Institution: Matsumoto University
• Principal Investigator: Kawnao Fuminori 松本大学, 大学院 健康科学研究科, 准教授 (90346156)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 骨格筋 / エピジェネティクス / 運動 / 遺伝子応答 / ヒストン

Outline of Final Research Achievements

The present study performed the series of experiments to demonstrate exercise-induced histone turnover in skeletal muscle. The experiment using H2B-GFP mice showed that 4 weeks of running training enhanced the incorporation of H2B-GFP fusion protein into the nucleosomes of myonuclei. In this muscle, the response of gene expression to single bout of exercise was drastically enhanced compared to that at 2 weeks in which histone turnover was not activated. It was also demonstrated that myonuclear expression of H2A-H2B-specific chaperone SPT16 was upregulated in association with the activation of histone turnover. These results demonstrate that exercise training activates the histone turnover in skeletal muscle fibers.

Free Research Field

骨格筋生理学

Academic Significance and Societal Importance of the Research Achievements

習慣的な運動は骨格筋においてヒストンターンオーバーを活性化するという新たな運動効果を見出した。ヒストンターンオーバーは遺伝子構造をオープンにするだけでなく、集積したヒストン修飾をリセットすることも想定される。運動には遺伝子基盤を変化させ、骨格筋の適応力そのものを改善する役割があることを一般社会に提唱できる。