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2022 Fiscal Year Final Research Report

Searching of nuclear receptor-mediated persistent unknown contaminants by an integrated approach of chemical analysis and bioassays

Research Project

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Project/Area Number 19K22911
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 63:Environmental analyses and evaluation and related fields
Research InstitutionEhime University

Principal Investigator

Kunisue Tatsuya  愛媛大学, 沿岸環境科学研究センター, 教授 (90380287)

Project Period (FY) 2019-06-28 – 2023-03-31
Keywords残留性未知物質 / バイオアッセイ / 機器分析 / 核内受容体 / 生物蓄積
Outline of Final Research Achievements

This study evaluated aryl hydrocarbon receptor (AhR)-agonistic activities in sulfuric acid (H2SO4)- or gel permeation chromatography (GCP)-treated liver extracts from 5 wild avian species by using in vitro bioassays. AhR-agonistic activities were observed in all the liver samples, especially with high activities in raptors such as falcon and black kite. The AhR-agonistic activities in GCP-treated liver extracts were higher than those in H2SO4-treated liver extracts, suggesting the presence of acid-labile AhR agonist(s). HPLC (high performance liquid chromatography)-fractionation and instrumental analysis approach indicated the accumulation of not only dioxins but also PAH (polycyclic aromatic hydrocarbon)-related compounds, as possible AhR agonists, in the liver of Japanese wild birds.

Free Research Field

環境化学

Academic Significance and Societal Importance of the Research Achievements

本研究で構築したin vitroバイオアッセイと機器分析のアプローチ手法は、生物蓄積性を示す未知有害化学物質を探索する上で有用なツールとなり得る。本手法により、日本に棲息する野生鳥類の肝臓にダイオキシン類や多環芳香族炭化水素といった既知のAhRアゴニストだけでなく、AhRアゴニスト活性を示す未同定化合物が蓄積していることを示唆した研究成果は学術的にも重要である。従来のターゲット分析では見落とされていた生態リスクが懸念される生物蓄積性化合物の可能性が高いことから、残留性有機汚染物質に関するストックホルム条約等の要監視化学物質として検討すべき基礎データを提示することができた。

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Published: 2024-01-30  

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