2020 Fiscal Year Final Research Report
Continuous generation of single-cell-laden microgels using micro pillar arrays
| Project/Area Number |
19K23585
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0402:Nano/micro science, applied condensed matter physics, applied physics and engineering, and related fields
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| Research Institution | Kyushu University (2020)
Tokyo Institute of Technology (2019) |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | マイクロナノデバイス / ゲル微粒子 / 粒子分離 |
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| Outline of Final Research Achievements |
In this study, we demonstrated separation of single-cell-encapsulated droplets, and continuous generation of cell-laden microgels using micropillar arrays. Water-in-oil droplets containing cells were produced in a microchannel and subsequently introduced into the micorpillar arrays to separate cell-encapsulated droplets from empty droplets. In addition, spherical cell-laden microgel particles dispersed in aqueous phase without satellite droplets were obtained by transporting precusor main droplets across the streams of the reaction solution and washing aqueous solution using micropillar arrays.
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| Free Research Field |
マイクロ流体デバイス
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| Academic Significance and Societal Importance of the Research Achievements |
従来のマイクロ流路を用いた単一細胞包埋ゲル微粒子の作製手法では，単一細胞の包埋効率は低く，また，ゲル微粒子の作製工程において，煩雑なバッチ式作業による，粒子洗浄・溶液置換が必要であるといった課題がある．対して本研究は，単一細胞包埋ゲル微粒子を迅速，簡便かつ高効率に作製可能な手法の確立を目指す，前例のない研究であり，提案するプロセス技術は，医学・生化学などの基礎研究，再生医療への応用など，新しい分野の開拓・発展を支える基盤技術になり得ると考えられる．
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