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2021 Fiscal Year Final Research Report

Design of artificial redox enzyme for biofuel cell

Research Project

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Project/Area Number 19K23664
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0601:Agricultural chemistry and related fields
Research InstitutionUniversity of Fukui

Principal Investigator

Takamura eiichiro  福井大学, 学術研究院工学系部門, 講師 (30843015)

Project Period (FY) 2019-08-30 – 2022-03-31
Keywordsバイオ電池 / 酸化還元酵素 / アノード / 直接電子移動 / 人工酸化還元酵素
Outline of Final Research Achievements

In the present study, we aimed to convert pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) from hyperthermophilic archaeon Pyrobaculum aerophilum, which has long-term stability, into a direct electron-transfer enzyme and to improve its electron transfer ability. The electron-transfer pathway in the wild type was inferred by evaluating mutants lacking lysine residues around PQQ by applying a method of modifying low-molecular-weight redox substances to lysine residues on the surface of the enzyme molecule. The biofuel cell using mutant PQQ-GDH exhibited 1.5-fold higher maximum power than the biofuel cell using wild-type.

Free Research Field

生物電気化学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果の学術的意義は次の2点である。(1)直接電子移動型酵素化したPQQ-GDHにおける電子伝達経路を明らかにした。(2)酸化還元部位-電極間電子伝達経路の特定の部位へ電子メディエータの新規修飾部位を追加することで、直接電子移動型酵素の電子伝達能を向上させられることを明らかにした。この成果は、現在盛んになってきているバイオ電池や生体センサ用酵素の開発に向けた、酵素分子設計指針の一つとして社会的意義は高い。

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Published: 2023-01-30  

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