2020 Fiscal Year Final Research Report
Molecular dissection of fruit internal tissue development
| Project/Area Number |
19K23672
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0602:Agricultural and environmental biology and related fields
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| Research Institution | Tokyo University of Agriculture and Technology (2020)
University of Tsukuba (2019) |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | 果実発達 / トマト / ゲノム編集 / CRISPRスクリーニング / イメージング質量分析 |
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| Outline of Final Research Achievements |
The regulatory mechanisms for the development of fruit internal tissues have not been well understood. To unveil this, we analyzed genes and metabolites potentially associated with internal tissue development of tomato fruit, as a model material. A CRISPR screening for previously identified 20 candidate genes revealed that a TALE family transcription factor gene is involved in the development of fruit internal tissue. A mass spectrometry imaging analysis using a mutant showing deficient growth of fruit internal tissues suggested that malic acid accumulation is associated with the internal tissue development in tomato fruit.
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| Free Research Field |
植物分子育種
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| Academic Significance and Societal Importance of the Research Achievements |
果実は食生活・食文化において欠かすことのできない作物であり，ヒトの嗜好性や健康に寄与する様々な有用成分の供給源となっている．本研究では，様々な有用成分を多く蓄積することが知られるトマト果実内部のゼリー組織に着目し，その発達に関連した遺伝子や代謝産物を明らかにした．本研究の成果は，様々な用途に応じた果実構造のデザインや，果実成分の蓄積場所として特定組織量の増大を可能とする新たな分子育種技術の開発につながることが期待される．
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