2020 Fiscal Year Final Research Report
Development of a novel SNP genotyping system for geneti management of farmed red sea bream
| Project/Area Number |
19K23687
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0603:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | 一塩基多型 / マダイ / 個体識別 / 次世代シーケンス / マルチプレックス |
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| Outline of Final Research Achievements |
In this study, genome-wide single nucleotide polymorphisms (SNPs) were obtained from five cultured red sea bream populations by GRAS-Di analysis. We obtained 187,568 to 98,047 SNPs from analysed populations, and selected 2,536 SNPs common to all populations by filtering based on missing rate, minor allele frequency, and deviation from Hardy-Weinberg law. In addition, we selected only SNPs that were more than 1 Mbp apart to avoid linkage disequilibrium, and finally 255 SNPs were selected. We designed primers for amplify these SNPs and developed a multiplex PCR system
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| Free Research Field |
水産遺伝育種
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| Academic Significance and Societal Importance of the Research Achievements |
本研究によりマダイの個体識別に用いるSNPパネルを開発することができたが、これにより、従来のマイクロサテライトDNA解析よりも安価かつ迅速に多数のマーカーによる遺伝子型判別が可能になると考えられる。本技術は、親子鑑定や系統識別に用いることが期待される。そのため、養殖現場では有用形質を持った親魚の選抜に威力を発揮することが期待されるとともに、養殖場から逸出した養殖マダイ個体の識別にも利用可能である。
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