Understanding the capacitation of naive embryonic stem cells in rat

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23711
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0605:Veterinary medical science, animal science, and related fields
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: Oikawa Mami 生理学研究所, 行動・代謝分子解析センター, 特任研究員 (30732903)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: ラット / ナイーブ型幹細胞

Outline of Final Research Achievements

We optimized culture conditions for differentiation of epiblast-like cells from embryonic stem cells in rat. EpiLCs showed similar gene expression profiles to that of in vivo epiblast. Furthermore, EpiLCs were able to differentiate into primordial germ cells like cells (PGCLCs). These results showed that we succeeded in differentiating functional rat EpiLCs.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

ナイーブ型幹細胞が多能性を獲得する機序を理解することは、効率的な細胞分化誘導系を確立する上で重要である。本研究で確立した、ラットES細胞-エピブラスト様細胞-始原細胞への分化誘導系は、ヒトを含む、他の動物種の多能性幹細胞を用いた細胞分化誘導系の確立や改良に役立つことが期待される。