2020 Fiscal Year Annual Research Report
Atomistic mechanism of the oligomerization of p53 protein in DNA scanning
| Project/Area Number |
19K23721
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TRAN PHUOC・DUY 東京工業大学, 生命理工学院, 助教 (50848546)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | p53 protein / A2a receptor / eePaCS-MD |
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| Outline of Annual Research Achievements |
We have successfully performed the dissociation of the p53 core domain tetramers out of the complex with DNA. We found that the bending of DNA contributes largely to the dissociation of the core domain out of the tetramers. We have successfully carried out the C-terminal domain scanning of DNA and find key interaction that contributes to the binding. We developed the method to sample efficiently the slow domain motion of the protein using PCA analysis and Markov State Modeling. We successfully constructed the full length complex of A2a receptor bound to Gs protein. The structure is stable and we found the mechanism of signal transmission pathway in the A2a/Gs protein complex.
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