2020 Fiscal Year Research-status Report
Physiological role of primary cilium-derived extracellular vesicles in fine-tuning signal transduction in target cells.
| Project/Area Number |
19K23728
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| Research Institution | Hiroshima University |
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Principal Investigator |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
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| Project Period (FY) |
2019-08-30 – 2022-03-31
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| Keywords | Primary Cilium / Extracellular Vesicles |
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| Outline of Annual Research Achievements |
The aim of this study is to find the physiological role of primary cilium derived extracellular vesicles in acceptor cells. Proteomics analysis of the target cells after exposure to pcEVs was performed. The results showed the increase in proteins related to cell proliferation and metabolism in the target cells exposed to pcEVs which were in line with our previous findings of increased rate of migration in target cells after exposure to pcEVs. Furthermore, in order to know whether the above mentioned effects were truly from pcEVs, we optimized the method to purify pcEVs from the mixture of extracellular vesicles using exosome depletion and purification of pcEVs.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
the research project is going rather smoothly as we have already devised the method to purify pcEVs which is important for confirming the physiological effects of pcEVs in target cells.
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| Strategy for Future Research Activity |
The next plan is to do the proteomics and RNA analysis of the purified pcEVs using our recently optimized purification methods and confirmation of the results through biochemical and biological assays.
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| Causes of Carryover |
Due to the pandemic situation some aspects of the research project could not be done. The remaining amount will be used for final omics analysis and the confirmation of the omics results and for paper publication costs.
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