2021 Fiscal Year Annual Research Report
Physiological role of primary cilium-derived extracellular vesicles in fine-tuning signal transduction in target cells.
| Project/Area Number |
19K23728
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| Research Institution | Hiroshima University |
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Principal Investigator |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
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| Project Period (FY) |
2019-08-30 – 2022-03-31
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| Keywords | Primary Cilium / Extracellular vesicles |
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| Outline of Annual Research Achievements |
The aim of this study is to find the physiological role of primary cilium derived extracellular vesicles in acceptor cells. We devised a filtration method to separate and purify primary cilium derived extracellular vesicles from exosomes. Next, the compatibility of the proteomics analysis with the devised pcEVs purification method was tested. The results showed that the method was not fully compatible with the subsequent proteomics analysis due to subsequent bovine serum albumin contamination. Subsequently, the extraction methods of pcEVs from filter after purification was changed for further analysis.
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Research Products
(1 results)
