2021 Fiscal Year Final Research Report
A DOF-CLE circuit that regulates phloem pattern in the Arabidopsis root
| Project/Area Number |
19K23750
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0702:Biology at cellular to organismal levels, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
QIAN PINGPING 大阪大学, 理学研究科, 助教 (20840466)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | DOF transcription factor / CLE peptide / LRR-RLK receptor / BAM / CIK / vasculature / phloem / cell patterning |
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| Outline of Final Research Achievements |
The regulatory mechanisms of phloem development are being uncovered. We find that a series of phloem-enriched DOF transcription factors (P-Dofs) not only regulate the number of procambium cell files, but also are necessary and sufficient for phloem differentiation. Overexpression of phloem-Dofs induced cells that expressed either sieve element or companion cell marker genes, which are mutually exclusive. Conversely, disruption of phloem-Dofs caused loss of phloem. Phloem-Dofs induce CLE25/26/45 peptides, which in turn inhibit expression of phloem-Dofs and phloem formation, forming a negative feedback loop. Disruption of multiple genes for either phloem-expressed CLEs, BAM-class receptors, or their coreceptors, CIKs, caused excess formation of phloem cell files. We further show that P-Dofs are under positive self and mutual regulation. These positive and negative feedback loops create the proper phloem pattern. There are more redundant DOFs, CLEs, and BAMs/CIKs involved in this pathway.
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| Free Research Field |
plant science
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| Academic Significance and Societal Importance of the Research Achievements |
Our results close a major gap in our understanding of phloem development in root primary growth.It is a good start for further studying the molecular mechanism of whole process of root vascular development and patterning.It also has strong theoretical guiding significance for future crop breeding.
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